Applications
MACS GMP Recombinant Human IL-12 can be used for a variety of applications, including:
Optimization of
ex vivo expansion protocols for adoptive cell transfer. Studies have demonstrated that
ex vivo activation of T cells in the presence of IL-12 can improve expansion and effector function and prevent
in vivo T cell exhaustion in animal models.
1Generation of cytokine-induced memory-like natural killer cells (CIML NK). Memory-like NK cells can be generated
ex vivo by cultivating human NK cells with a combination of IL-12, IL-15 and IL-18. Such CIML NK cells demonstrated an increased longevity and enhanced anticancer functionality compared to normal effector NK cells.
2In vitro differentiation of naive CD4
+ T cells towards Th1 cells. After activation, naïve CD4
+ T cells can be differentiated into Th1 effector cells when cultured with a combination of IL-2, IL-12 and IFNγ.
3 Technical specificationsMACS GMP Recombinant Human IL-12 is manufactured and released based on the stringent specifications displayed below.
Lot-specific results of the respective QC measurements are depicted on each Product Quality Certificate (PQC). Learn more
here.- Identity: Confirmed by isoelectric focusing and chip electrophoresis. This corresponds to the mature form of IL-12.
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Product-related proteins: ≤15% as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for CHO genomic DNA.
- Residual host cell protein content: < 25 µg/mg, as determined by CHO HCP ELISA.
MACS GMP Recombinant Human IL-12 is expressed as a single chain fusion protein. It has been shown that such fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro and
in vivo.
5 The product is lyophilized without carrier protein or preservatives.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.