Name: MACS GMP Recombinant Human IL-12
Availability: InStock

Product data and images for MACS GMP Recombinant Human IL-12

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The biological activity of MACS GMP Recombinant Human IL-12 was determined by induction of IFN-γ secretion by PHA-activated T cells.

Specifications for MACS GMP Recombinant Human IL-12

Overview

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

MACS

^®

GMP Recombinant Human IL-12 is designed for

ex vivo

cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells.

IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations.

Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the TH 1 rather than the TH 2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity.

It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive

in vitro

and in

vivo

^2-4

Applications

Human IL-12 can be used for a variety of applications, including:

In vitro differentiation of naive CD4⁺ T cells towards TH 1 cells.
In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells

Biological activity

The specific activity is determined by induction of IFN-γ secretion by PHA-activated T cells.
¹
The proliferation assay was calibrated with the reference reagent for human IL-12 (NIBSC code 95/544) provided by the WHO/National Institute for Biological Standards and Control.
Specific activity: ≥ 3×
10
⁶
U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and

ex vivo

cell culture processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources for MACS GMP Recombinant Human IL-12

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for MACS GMP Recombinant Human IL-12

Publications

Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
Lieschke, G.J. et al. (1997)
Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
in vivo.
Nat. Biotechnol. 15: 35-40
Miller, J.M. et al. (2010)
Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
in vitro
and
in vivo.
Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
Juelke, K. et al. (2010)
CD62L expression identifies a unique subset of polyfunctional CD56
^dim
NK cells.
Blood 116(8): 1299-1307

Data and images

Data and images for MACS GMP Recombinant Human IL-12

Figures

Figure

View details

The biological activity of MACS GMP Recombinant Human IL-12 was determined by induction of IFN-γ secretion by PHA-activated T cells.

Specifications

Specifications for MACS GMP Recombinant Human IL-12

Overview

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

MACS

^®

GMP Recombinant Human IL-12 is designed for

ex vivo

Detailed product information

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells.

It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive

in vitro

and in

vivo

^2-4

Applications

Human IL-12 can be used for a variety of applications, including:

In vitro differentiation of naive CD4⁺ T cells towards TH 1 cells.
In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells

Biological activity

The specific activity is determined by induction of IFN-γ secretion by PHA-activated T cells.
¹
The proliferation assay was calibrated with the reference reagent for human IL-12 (NIBSC code 95/544) provided by the WHO/National Institute for Biological Standards and Control.
Specific activity: ≥ 3×
10
⁶
U/mg

Quality statement

Disclaimer

MACS GMP Products are for research use and

ex vivo

cell culture processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Resources

Resources for MACS GMP Recombinant Human IL-12

Documents and Protocols

Brochures/posters

MACS® GMP Cytokines - Culture is key

App notes/customer reports

MACS® GMP Cytokines - Cytokine stability studies

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References

References for MACS GMP Recombinant Human IL-12

Publications

Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
Lieschke, G.J. et al. (1997)
Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
in vivo.
Nat. Biotechnol. 15: 35-40
Miller, J.M. et al. (2010)
Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
in vitro
and
in vivo.
Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
Juelke, K. et al. (2010)
CD62L expression identifies a unique subset of polyfunctional CD56
^dim
NK cells.
Blood 116(8): 1299-1307

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MACS GMP Recombinant Human IL-12

MACS GMP Recombinant Human IL-12

Product overview

Product data and images for MACS GMP Recombinant Human IL-12

Figures

Figure

Specifications for MACS GMP Recombinant Human IL-12

Overview

Detailed product information

Background information

Applications

Biological activity

Quality statement

Disclaimer

Resources for MACS GMP Recombinant Human IL-12

Documents and Protocols

Certificates

References for MACS GMP Recombinant Human IL-12

Publications

Data and images for MACS GMP Recombinant Human IL-12

Figures

Figure

Specifications for MACS GMP Recombinant Human IL-12

Overview

Detailed product information

Background information

Applications

Biological activity

Quality statement

Disclaimer

Resources for MACS GMP Recombinant Human IL-12

Documents and Protocols

Certificates

References for MACS GMP Recombinant Human IL-12

Publications

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