IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS GMP Recombinant Human IL-12 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human IL-12

Figures

Figure 1

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MACS GMP Recombinant Human IL-12 biological activity.
Activity of Human MACS GMP IL-12 (blue bar), was compared to another commercially available product (black bar).

Figure 1

MACS GMP Recombinant Human IL-12 biological activity.
Activity of Human MACS GMP IL-12 (blue bar), was compared to another commercially available product (black bar).

Figure

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The biological activity of MACS GMP Recombinant Human IL-12 was determined by induction of IFN-γ secretion by PHA-activated T cells.
The biological activity of MACS GMP Recombinant Human IL-12 was determined by induction of IFN-γ secretion by PHA-activated T cells.

Specifications for
MACS
®
GMP Recombinant Human IL-12

Overview

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS GMP Recombinant Human IL-12 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells.
IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations.
Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T
H
1 rather than the T
H
2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity.
It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and in
vivo
.
2-4

Applications

Human IL-12 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH1 cells.
  • In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells

Biological activity

  • The specific activity is determined by induction of IFN-γ secretion by PHA-activated T cells.
    1
    The proliferation assay was calibrated with the reference reagent for human IL-12 (NIBSC code 95/544) provided by the WHO/National Institute for Biological Standards and Control.
  • Specific activity: ≥ 3×
    10
    6
    U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human IL-12

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human IL-12

Publications

  1. Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
  2. Lieschke, G.J. et al. (1997)
    Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
    in vivo.
    Nat. Biotechnol. 15: 35-40
  3. Miller, J.M. et al. (2010)
    Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
    in vitro
    and
    in vivo.
    Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
  4. Juelke, K. et al. (2010)
    CD62L expression identifies a unique subset of polyfunctional CD56
    dim
    NK cells.
    Blood 116(8): 1299-1307

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