Fibroblast growth factor 2 (FGF-2), also termed basic FGF, belongs to the family of heparin-binding growth factors. FGF-2 functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells.
MACS GMP Recombinant Human FGF-2 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or

Data and images for
MACS
®
GMP Recombinant Human FGF-2

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human FGF-2.
The peaks correspond to the mature form of human FGF-2, both with the N-terminal proline (146 amino acids) and without the N-terminal proline (145 amino acids), respectively.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human FGF-2.
The peaks correspond to the mature form of human FGF-2, both with the N-terminal proline (146 amino acids) and without the N-terminal proline (145 amino acids), respectively.

Figure 2

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MACS GMP Recombinant Human FGF-2 biological activity.
Activity of Human FGF-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human FGF-2 biological activity.
Activity of Human FGF-2, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

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MACS GMP Recombinant Human FGF-2 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP FGF-2.

Figure 3

MACS GMP Recombinant Human FGF-2 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP FGF-2.

Specifications for
MACS
®
GMP Recombinant Human FGF-2

Overview

Fibroblast growth factor 2 (FGF-2), also termed basic FGF, belongs to the family of heparin-binding growth factors. FGF-2 functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells.
MACS GMP Recombinant Human FGF-2 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Applications

MACS GMP Recombinant Human FGF-2 can be used for a variety of applications, including the culture of undifferentiated human embryonic stem cells
2,3
or the expansion of mesenchymal stromal cells
4,5
.

Biological activity

  • The specific activity is determined by proliferation assay according to Robinson and Gaines-Das
    1
    using 3T3 cells. The proliferation assay was calibrated with the international standard for human FGF-2 (NIBSC code 90/712) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 4×
    10
    5
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human FGF-2

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human FGF-2

Publications

  1. Robinson, C. J. and Gaines-Das, R. (1994)
    The international standard for basic fibroblast growth factor (FGF-2); comparison of candidate preparations by
    in vitro
    bioassays and immunoassays.
    Growth Factors 11: 9-16
  2. Levenstein, M. E. et al. (2006) Basic fibroblast growth factor support of human embryonic stem cell self-renewal. Stem Cells 24: 568-574
  3. Xu, C. et al. (2005) Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. Stem Cells 23: 315-323
  4. Ito et al. (2008) FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-beta signaling. Cytotechnology 56: 1-7
  5. Solchaga, L. A. et al. (2005) FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells. J. Cell. Physiol. 203: 398-409

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®
GMP Recombinant Human FGF-2

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