The MACS GMP PepTivator HCMV pp65 is a peptide pool that consists mainly of 15-mer peptides with eleven amino-acids overlap. It has been developed for efficient
in vitro
stimulation and subsequent isolation of HCMV pp65-specific CD4
+
and CD8
+
T cells.

Specifications for
MACS
®
GMP PepTivator
®
HCMV pp65

Overview

The MACS GMP PepTivator HCMV pp65 is a peptide pool that consists mainly of 15-mer peptides with eleven amino-acids overlap. It has been developed for efficient
in vitro
stimulation and subsequent isolation of HCMV pp65-specific CD4
+
and CD8
+
T cells.

Detailed product information

Background information

Human cytomegalovirus (HCMV) is a member of the herpes virus group and belongs to the subfamily of beta-herpes viruses. Between 50 and 85% of human adults are infected with HCMV. Once infected, the virus persists in the organism. The infection is asymptomatic in healthy individuals but in immunocompromized patients HCMV can cause severe disease.
The pp65 protein (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. pp65 is an immunodominant target of CD4
+
as well as CD8
+
T cell responses to HCMV.

Applications

Antigen-specific T cells play a critical role in the regulation of immune responses and the elimination of virus-infected or malignant cells in the human body. Adoptive transfer of clinical-grade pp65-specific CD4
+
and CD8
+
T cells is a valuable tool for the treatment of HCMV infections after hematopoietic stem cell transplantation (SCT)
1,2
and shows potential for solid organ transplantation (SOT)
3
.
The
in vitro
stimulation of pp65-specific CD4
+
and CD8
+
T cells with MACS
®
GMP PepTivator HCMV pp65 causes the production of the effector cytokine IFN-gamma. The secretion of IFN-gamma then permits the enrichment of pp65-specific effector/memory T cells with the use of the CliniMACS Cytokine Capture System (IFN-gamma).

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP PepTivator
®
HCMV pp65

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP PepTivator
®
HCMV pp65

Publications

  1. Einsele et al. (2002) Infusion of cytomegalovirus (CMV)-specific T cells for the treatment of CMV infection not responding to antiviral chemotherapy. Blood 99: 3916-3922
  2. Mackinnon et al. (2008) Adoptive cellular therapy for cytomegalovirus infection following allogeneic stem cell transplantation using virus-specific T cells. Blood Cells Mol. Dis. 40: 63-67
  3. Brestrich, G. et al. (2009) Adoptive T-cell therapy of a lung transplanted patient with severe CMV disease and resistance to antiviral therapy. Am. J. Transplant. 9(7): 1679-84

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®
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