MACS GMP CpG-P can be used to activate immune cells, in particular human B cells and plasmacytoid dendritic cells (PDCs).

Specifications for
MACS
®
GMP CpG-P

Overview

MACS GMP CpG-P can be used to activate immune cells, in particular human B cells and plasmacytoid dendritic cells (PDCs).

Detailed product information

Background information

The toll-like-receptor (TLR) 9 is a prominent member of the TLR family recognizing pathogen-associated molecular patterns. TLR 9 is expressed in particular on the surface of plasmacytoid dendritic cells (PDCs) and B cells and binds specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells.
These effects can be mimicked by short synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs. Several classes of CpG ODNs have been identified and can be distinguished by their effects on certain cell types with regard to stimulation and cytokine production.
The recently discovered P-class ODNs induce a strong IFN-α production as well as cell activation and thus show similar but superior properties to C-class ODNs.

Applications

MACS GMP CpG-P can be used to activate immune cells, in particular human B cells and plasmacytoid dendritic cells (PDCs).

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP CpG-P

Certificates

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References for
MACS
®
GMP CpG-P

Publications

  1. Hemmi, H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
  2. Krieg, A. M. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
  3. Bauer, S. et al. (2001) Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. U.S.A. 98: 9237-9242
  4. Vollmer J. et al. (2004) Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities Eur. J. Immunol. 34: 251-262
  5. Krug et al. (2001) Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037
  6. Donhauser, N. et al. (2010) Differential effects of P‑class versus other CpG oligodeoxynucleotide classes on the impaired innate immunity of plasmacytoid dendritic cells in HIV type 1 infection. AIDS Res Hum Retroviruses 26(2): 161-171
  7. Vollmer, J. and A. M. Krieg (2009) Immunotherapeutic applications of CpG oligodeoxynucleotide TLR9 agonists. Adv. Drug Deliv. Rev. 61: 195-204
  8. Samulowitz, U. et al. (2010) A novel class of immune-stimulatory CpG oligodeoxynucleotides unifies high potency in type I interferon induction with preferred structural properties. Oligonucleotides 20: 93-101

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