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Human PBMCs were incubated with or without PepTivator CMV pp65 for 6 hours; brefeldin A was added after 2 hours. The cells were stained with the CD4 T Cell Detection Cocktail and Rapid Cytokine Inspector Anti-IFN-γ-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. IFN-γ and CD154 expression was analyzed for CD4+
T cells in stimulated and unstimulated samples*.
*Please refer to the data sheet for detailed gating strategy and analysis.
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