CD154/IFN-γ/CD4 Detection Kit, human

CD154/IFN-γ/CD4 Detection Kit, human

The kit is suitable for flow cytometric identification and enumeration of IFN-γ-producing CD154
+
CD4
+
T cells upon
in vitro
stimulation. Other applications include identification and enumeration of IFN-γ-producing, antigen-specific CD154
+
CD4
+
T cells upon restimulation or monitoring of specificity of antigen-specific T cell lines.

Background information

The CD154/IFN-γ/CD4 Detection Kit is designed for the intracellular detection of IFN-γ production in activated CD4
+
T cells. It contains an antibody recognizing CD154 – a specific marker for T cell activation – and all reagents necessary for the immunofluorescent detection of IFN-γ-producing activated CD4
+
T cells from whole blood or PBMCs.
By combining the detection of cytokine production with staining of CD154 and CD4, the sensitive and specific detection of activated antigen-specific CD4
+
T cells is easily possible.
IFN-γ is predominantly produced by activated CD4
+
and CD8
+
memory/effector T cells and by NK cells. The quantitative analysis of IFN-γ-producing cells can provide important information on the course of antigen-specific immune responses.
Product options: 1

Product information

Size
Order no.
Price

CD154/IFN-γ/CD4 Detection Kit, human

  • For research use only
for 100
tests
(1)
130-092-814
EUR 1.122,00