REAlease® Anti-Sca-1 Complexes, mouse

REAlease
®
Anti-Sca-1 Complexes
, mouse

Clone: REAL175 | Dilution: 1:50
Clone REAL175 is an antibody fragment derived from the full Anti-Sca-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL175 recognizes the mouse stem cell antigen-1 (Sca-1) antigen, an 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REAL175 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit
+
Sca-1
+
Lin
) cells. In combination with CD105 (Endoglin) expression, Sca-1 has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow. In addition, Sca-1
+
bone marrow cells also contain the mesenchymal stem cell fraction. Sca-1
+
cells have been shown to give rise to hepatocytes
in vivo
and neural cells
in vitro
. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative Names

LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Technical specifications

  • Antigen: Sca-1
  • Clone: REAL175
  • Isotype control: REAL Control
  • Alternative names of antigen: LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
  • Distribution of antigen: hematopoietic stem cells, hemotopoietic progenitor cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC
Product options: 2

Product information

Size
Order no.
Price

REAlease Anti-Sca-1-FITC, mouse

  • REAL175
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-115-429
EUR 50,00 

REAlease Anti-Sca-1-FITC Kit, mouse

  • REAL175
  • 1:50
  • For research use only
for 100
tests
130-122-615
EUR 175,00 

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