REAlease® Anti-Ly-49E/F Complexes, mouse

REAlease
®
Anti-Ly-49E/F Complexes
, mouse

Clone: REAL331 | Dilution: 1:50
Clone REAL331 is an antibody fragment derived from the full Anti-Ly-49E/F antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL331 recognizes the mouse killer cell lectin-like receptor 5 and 6 (Ly49E and Ly49F) antigens, which are type II transmembrane natural killer cell receptors belonging to the Ly49 family. Members of the Ly49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly49E is expressed abundantly on fetal or immature natural killer (NK) cells and on certain γδ T cells, but at barely detectable frequencies on adult splenic NK cells. Different from other Ly49 receptors, Ly49E contains an immunoreceptor tyrosine-based inhibitory motif domain, a charged transmembrane residue and it is present in all mouse strains. Its failure to bind to a variety of soluble or cell bound MHC class I molecules indicates that its natural ligand is not an endogenous class I molecule. Interleukin 2 (IL-2) and IL-15 selectively induce the
de novo
expression of Ly49E on the majority of mature NK cells. By contrast, IL-4 blocks this IL-2 induced acquisition of Ly49E. Ly49F is expressed on subsets of NK cells, NK-T cells, and on a subpopulation of memory T cells in C57BL/6 mice, but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice. IL-21 selectively down-regulates Ly49F on mature NK cells.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative Names

Klra5, Ly49e, Ly49F, Klra6

Technical specifications

  • Antigen: Ly-49E/F
  • Clone: REAL331
  • Isotype control: REAL Control
  • Alternative names of antigen: Klra5, Ly49e, Ly49F, Klra6
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE
Product options: 2

Product information

Size
Order no.
Price

REAlease Anti-Ly-49E/F-PE, mouse

  • REAL331
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-118-530
EUR 105,00 

REAlease Anti-Ly-49E/F-PE Kit, mouse

  • REAL331
  • 1:50
  • For research use only
for 100
tests
130-122-612
EUR 175,00