CD8a antibodies, mouse

CD8a antibodies, mouse

Clone: REA601 | Dilution: 1:10
Clone REA601 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ
+
cells. CD8a is further detected on most thymocytes (CD4
+
CD8a
+
and CD4
CD8
+
thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ
+
cells express a CD8a/CD8a homodimer. It mediates efficient cell-cell interactions within the immune system.
Additional information: Clone REA601 displays negligible binding to Fc receptors.

Alternative Names

CD8, Leu-2, Ly-2, T8, Lyt-2

Technical specifications

  • Antigen: CD8a
  • Clone: REA601
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD8, Leu-2, Ly-2, T8, Lyt-2
  • Entrez Gene ID: 12525
  • Molecular mass of antigen [kDa]: 25
  • Distribution of antigen: dendritic cells, NK cells, red blood cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, VioGreen, PE-Vio615, APC-Vio770

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA601
53-6.7++
Product options: 10
130-109-556

CD8a-VioBright FITC, mouse

9 µg in 300 µL (1)
EUR 55,00 
130-109-477

CD8a-VioBright FITC, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-109-325

CD8a-PE, mouse

9 µg in 300 µL (1)
EUR 39,00 
130-109-247

CD8a-PE, mouse

30 µg in 1 mL (1)
EUR 60,00 
130-109-330

CD8a-VioGreen, mouse

9 µg in 300 µL (1)
EUR 81,00 
130-109-252

CD8a-VioGreen, mouse

30 µg in 1 mL (1)
EUR 95,00 
130-109-329

CD8a-PE-Vio615, mouse

9 µg in 300 µL (1)
EUR 67,00 
130-109-251

CD8a-PE-Vio615, mouse

30 µg in 1 mL (1)
EUR 100,00 
130-109-328

CD8a-APC-Vio770, mouse

9 µg in 300 µL (1)
EUR 71,00 
130-109-250

CD8a-APC-Vio770, mouse

30 µg in 1 mL (1)
EUR 100,00