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Splenocytes from Wistar rats were stained with CD49d antibodies or with the corresponding isotype control antibodies (left image) as well as with CD45R antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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