CD45 antibodies, human

CD45 antibodies, human

Clone: REA747 | Dilution: 1:50
Clone REA747 recognizes the human CD45 antigen, a member of the protein tyrosine phosphatase (PTP) family, also known as the leukocyte common antigen (LCA). The CD45 molecule is a type I transmembrane protein which is required for T and B cell activation and involved in cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 is expressed in different isoforms (CD45RA, CD45RB, CD45RC, CD45RAB, CD45RAC, CD45RBC, CD45RO, CD45R (ABC)) depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms.
Additional information: Clone REA747 displays negligible binding to Fc receptors.

Alternative Names

Ptprc, GP180, L-CA, LY5, T200

Technical specifications

  • Antigen: CD45
  • Clone: REA747
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Ptprc, GP180, L-CA, LY5, T200
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Distribution of antigen: B cells, basophils, dendritic cells, granulocytes, hematopoietic stem cells, Langerhans cells, leukocytes, lymphocytes, macrophages, mast cells, monocytes, plasma cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, VioBright 667, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA747
2D1++
HI30++
5B1++
MB4-6D6-
REA1023-
  • Selected References

    1. Ralph, S. J. et al. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen). EMBO J. 6(5): 1251-1257
    2. Vykoukal, J. et al. (2008) Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. Lab Chip 8(8): 1386-1393
    3. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83
Product options: 25

Product information

Size
Order no.
Price

CD45-FITC, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-769
EUR 70,00 

CD45-FITC, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-631
EUR 175,00 

CD45-FITC, human

  • REA747
  • 1:50
  • For research use only
for 500
tests
(1)
130-114-569
EUR 440,00 

CD45-PE, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-770
EUR 95,00 

CD45-PE, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-632
EUR 235,00 

CD45-APC, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-771
EUR 95,00 

CD45-APC, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-633
EUR 235,00 

CD45-VioBlue, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-775
EUR 115,00 

CD45-VioBlue, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-637
EUR 275,00 

CD45-VioGreen, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-776
EUR 115,00 

CD45-VioGreen, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-638
EUR 275,00 

CD45-PE-Vio615, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-777
EUR 120,00 

CD45-PE-Vio615, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-639
EUR 290,00 

CD45-PE-Vio770, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-772
EUR 120,00 

CD45-PE-Vio770, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-634
EUR 290,00 

CD45-APC-Vio770, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-773
EUR 120,00 

CD45-APC-Vio770, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-635
EUR 290,00 

CD45-PerCP-Vio700, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-774
EUR 120,00 

CD45-PerCP-Vio700, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-636
EUR 290,00 

CD45-VioBright 515, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-778
EUR 150,00 

CD45-VioBright 515, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-640
EUR 360,00 

CD45-VioBright 667, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-779
EUR 150,00 

CD45-VioBright 667, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-641
EUR 360,00 

CD45-Biotin, human

  • REA747
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-768
EUR 70,00 

CD45-Biotin, human

  • REA747
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-630
EUR 175,00