CD317 (PDCA-1) antibodies – functional grade, mouse

CD317 (PDCA-1) antibodies – functional grade, mouse

Clone: JF05-1C2.4.1
Clone JF05-1C2.4.1 recognizes the mouse CD317 antigen, also known as plasmacytoid dendritic cell antigen-1 (PDCA-1). CD317 is specifically expressed on mouse PDCs, a subset of CD11c
+
dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues.
In mouse spleen, bone marrow, and lymph nodes, PDCA-1 is exclusively expressed on cells which are CD11c
low
, Ly-6C
high
, CD45R (B220)
+
, MHC class II
low
, CD8a
low/–
, CD80
low/–
, CD86
, CD40
, CD11b
, CD90
, CD49b (DX5)
, CD3
, CD19
, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed that all CD11c
low
Ly-6C
high
CD45R (B220)
+
PDCs are PDCA-1
+
and that PDCA-1 expression is restricted to PDCs.

Applications

The CD317 (PDCA-1) pure – functional grade antibody is suited for
in vivo
depletion of PDCs for studies on their functional properties in experimental mouse models of infection and autoimmunity.

Alternative Names

BST2, Bst-2, C87040, CD317, DAMP-1, GREG

Technical specifications

  • Antigen: CD317 (PDCA-1)
  • Clone: JF05-1C2.4.1
  • Isotype: rat IgG2b
  • Alternative names of antigen: BST2, Bst-2, C87040, CD317, DAMP-1, GREG
  • Entrez Gene ID: 69550
  • Molecular mass of antigen [kDa]: 17
  • Distribution of antigen: dendritic cells
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected References

    1. Fischer et al. (2004) MACS&more 8(1): 13-15
    2. Krug, A. et al. (2004) TLR9-dependent recognition of MCMV by IPC and DC generates coordinated cytokine responses that activate antiviral NK cell function. Immunity 21: 107-119
    3. Yoneyama, H. et al. (2005) Plasmacytoid DCs help lymph node DCs to induce anti-HSV CTLs. J. Exp. Med. 202(3): 425-435
    4. Shen, H. and Iwasaki, A. (2006) A crucial role for plasmacytoid dendritic cells in antiviral protection by CpG ODN-based vaginal microbicide. J. Clin. Invest. 116: 2237-2243
    5. Kuwajima, S. et al. (2006) Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation. Nat. Immunol. 7: 740-746
Product options: 2

Product information

Size
Order no.
Price

CD317 (PDCA-1) pure - functional grade, mouse

  • JF05-1C2.4.1
  • For research use only
1 mg in 0.5 mL
130-091-978
EUR 583,00 

CD317 (PDCA-1) pure - functional grade, mouse

  • JF05-1C2.4.1
  • For research use only
8 mg in 4 mL
130-092-550
EUR 2.935,00