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Immature human monocyte-derived dendritic cells were stained with CD276 antibodies or with the corresponding isotype control antibodies (left peak). The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.