CD270 (HVEM) antibodies, mouse

Alternative Names

Technical specifications

• Antigen: CD270 (HVEM)
• Clone: REA275
• Isotype: recombinant human IgG1
• Isotype control: REA Control antibodies
• Alternative names of antigen: TNFRSF14, ATAR, HVEA, HVEM, Tnfrs14
• Entrez Gene ID: 230979
• Molecular mass of antigen [kDa]: 26
• Distribution of antigen: B cells, T cells, monocytes, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, PE-Vio770

• Figure 1

  Mouse splenocytes were stained with CD270 (HVEM) antibodies, as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected References

  1. Hsu, H. et al. (1997) ATAR, a novel tumor necrosis factor receptor family member, signal through TRAF2 and TRAF5. J. Biol. Chem. 272: 13471-13474
  2. Kwon, B. S. et al. (1997) A newly identified member of the tumor necrosis factor receptor superfamily with a wide tissue distribution and involvement in lymphocyte activation. J. Biol. Chem. 272: 14272-14276
  3. Kim, W. K. et al. (2012) Absence of herpes virus entry mediator (HVEM) increases bone mass by attenuating receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis. Endocrinology 153(10): 4808-4817