CD20 Cytoplasmic antibodies, human

CD20 Cytoplasmic antibodies, human

Clone: REA543 | Dilution: 1:11
Clone REA543 recognizes the cytoplasmic domain of the human CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca
2+
channel and might function in the regulation of local responses during B cell activation.
In vitro
effects of CD20-specific antibodies on resting B cells indicate that CD20 is able to transduce an extracellular signal affecting the G0/G1 cell cycle transition. Studies have demonstrated that CD20-initiated intracellular signals involve tyrosine kinase activation and that CD20 is tightly associated with both serine and tyrosine kinases.
Additional information: Clone REA543 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: CD20 Cytoplasmic
  • Clone: REA543
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Entrez Gene ID: 931
  • Molecular mass of antigen [kDa]: 33
  • Distribution of antigen: B cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700
  • Selected References

    1. Einfeld, D. A. et al. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. EMBO J. 7(3): 711-717
    2. Deans, J. P. et al. (1995) Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J. Biol. Chem. 270(38): 22632-22638
    3. Vaughan, A. T. et al. (2015) Activatory and inhibitory Fcγ receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain. J. Biol. Chem. 290(9): 5424-5437
Product options: 10

Product information

Size
Order no.
Price

CD20 Cytoplasmic-PE, human

  • REA543
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-313
EUR 110,00 

CD20 Cytoplasmic-PE, human

  • REA543
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-289
EUR 265,00 

CD20 Cytoplasmic-APC, human

  • REA543
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-314
EUR 110,00 

CD20 Cytoplasmic-APC, human

  • REA543
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-290
EUR 265,00 

CD20 Cytoplasmic-PE-Vio770, human

  • REA543
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-315
EUR 120,00 

CD20 Cytoplasmic-PE-Vio770, human

  • REA543
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-291
EUR 290,00 

CD20 Cytoplasmic-APC-Vio770, human

  • REA543
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-316
EUR 120,00 

CD20 Cytoplasmic-APC-Vio770, human

  • REA543
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-292
EUR 290,00 

CD20 Cytoplasmic-PerCP-Vio700, human

  • REA543
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-317
EUR 120,00 

CD20 Cytoplasmic-PerCP-Vio700, human

  • REA543
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-293
EUR 290,00