CD16 antibodies, human

CD16 antibodies, human

Clone: REA423 | Dilution: 1:50
Clone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.
Additional information: Clone REA423 displays negligible binding to Fc receptors.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Technical specifications

  • Antigen: CD16
  • Clone: REA423
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII
  • Entrez Gene ID: 2214, 2215
  • Molecular mass of antigen [kDa]: 48
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , capuchin monkey,
    chimpanzee (
    Pan troglodytes
    )
    ,
    common marmoset (
    Callithrix jacchus
    )
    ,
    cotton-top tamarin (
    Saguinus oedipus
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    pigtail monkey (
    Macaca nemestrina
    )
    ,
    squirrel monkey (
    Saimiri sciureus
    )
  • Distribution of antigen: NK cells, monocytes, granulocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin, VioBright 515, PE-Vio615
  • Selected references

    1. Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
    2. Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
    3. Ziegler-Heitbrock, L. (2007)
      The CD14
      +
      CD16
      +
      blood monocytes: their role in infection and inflammation.
      J. Leukoc. Biol. 584(592): 584-592
    4. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
    5. Veluchamy, J. et al. (2017)
      In vivo
      efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
      Front Immunol 8: 87
Product options: 22
130-113-954

CD16-FITC, human

for 30
tests
(1)
EUR 70,00 
130-113-392

CD16-FITC, human

for 100
tests
(1)
EUR 175,00 
130-113-955

CD16-PE, human

for 30
tests
(1)
EUR 95,00 
130-113-393

CD16-PE, human

for 100
tests
(1)
EUR 235,00 
130-113-951

CD16-APC, human

for 30
tests
(1)
EUR 95,00 
130-113-389

CD16-APC, human

for 100
tests
(1)
EUR 235,00 
for 30
tests
(1)
EUR 115,00 
for 100
tests
(1)
EUR 275,00 
for 30
tests
(1)
EUR 115,00 
for 100
tests
(1)
EUR 275,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 
for 30
tests
(1)
EUR 70,00 
for 100
tests
(1)
EUR 175,00 
for 30
tests
(1)
EUR 150,00 
for 100
tests
(1)
EUR 360,00 
for 30
tests
(1)
EUR 120,00 
for 100
tests
(1)
EUR 290,00 

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