CD11b antibodies, human

CD11b antibodies, human

Clone: REA713 | Dilution: 1:50
Clone REA713 recognizes the human CD11b antigen (Mac-1 α; integrin αM chain), which is part of the CD11b/CD18 heterodimer (Mac-1 α, Mβ2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X. In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes as well as on microglia in the brain.
Additional information: Clone REA713 displays negligible binding to Fc receptors.

Alternative Names

integrin αM, Mac-1 α, Integrin alpha-M, CR3A

Technical specifications

  • Antigen: CD11b
  • Clone: REA713
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: integrin αM, Mac-1 α, Integrin alpha-M, CR3A
  • Entrez Gene ID: 3684, 16409
  • Molecular mass of antigen [kDa]: 126
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    chimpanzee (
    Pan troglodytes
    )
    , baboon,
    common marmoset (
    Callithrix jacchus
    )
    , pig, porcine
  • Distribution of antigen: myeloid cells, NK cells, microglia, brain, lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA713 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA713
ICRF44++
M1/70-
M1/70.15.11.5+
CBRM1/5-
REA592-
  • Selected References

    1. Pahl, H. L. et al. (1992) Characterization of the myeloid-specific CD11b promoter. Blood 79(4): 865-870
    2. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J. Neurosci. 30(29): 9651-9658
    3. Liu, T. et al. (2014)
      Gr-1
      +
      CD11b
      +
      cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation.
      Sci. Rep. 4: 4833
Product options: 18
130-110-610

CD11b-FITC, human

for 30
tests
(1)
EUR 70,00 
130-110-552

CD11b-FITC, human

for 100
tests
(1)
EUR 175,00 
130-110-611

CD11b-PE, human

for 30
tests
(1)
EUR 95,00 
130-110-553

CD11b-PE, human

for 100
tests
(1)
EUR 235,00 
130-110-612

CD11b-APC, human

for 30
tests
(1)
EUR 95,00 
130-110-554

CD11b-APC, human

for 100
tests
(1)
EUR 235,00 
130-110-616

CD11b-VioBlue, human

for 30
tests
(1)
EUR 115,00 
130-110-558

CD11b-VioBlue, human

for 100
tests
(1)
EUR 275,00 
130-110-617

CD11b-VioGreen, human

for 30
tests
(1)
EUR 115,00 
130-110-559

CD11b-VioGreen, human

for 100
tests
(1)
EUR 275,00 
130-110-613

CD11b-PE-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-110-555

CD11b-PE-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-110-614

CD11b-APC-Vio770, human

for 30
tests
(1)
EUR 120,00 
130-110-556

CD11b-APC-Vio770, human

for 100
tests
(1)
EUR 290,00 
130-110-615

CD11b-PerCP-Vio700, human

for 30
tests
(1)
EUR 120,00 
130-110-557

CD11b-PerCP-Vio700, human

for 100
tests
(1)
EUR 290,00 
130-110-609

CD11b-Biotin, human

for 30
tests
(1)
EUR 70,00 
130-110-551

CD11b-Biotin, human

for 100
tests
(1)
EUR 175,00