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Jurkat cells were fixed, permeabilized, and stained with CD107a (LAMP-1) antibodies or with the corresponding REA Control (I) antibodies (left peak). Control antibodies are unstained in case of VioGreen™ and VioBright 515 fluorochromes. Flow cytometry was performed using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris were excluded from the analysis based on scatter signals.
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