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Jurkat cells were either left unstimulated (left peak) or stimulated with 5 mM H2
for 15 minutes. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-Syk pY348 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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