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Splenocytes from C57BL/6 mice either left unstimulated (left image) or stimulated with 100 ng/mL mouse IL-4 for 30 minutes, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-STAT6 pY641 antibodies as well as with CD4 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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