Anti-Sca-1 antibodies, mouse

Anti-Sca-1 antibodies, mouse

Clone: REA422 | Dilution: 1:10
Clone REA422 recognizes the mouse stem cell antigen-1 (SCA-1) antigen, a 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REA422 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit
+
Sca-1
+
Lin
-
) cells. Sca-1 in combination with CD105 (Endoglin) expression has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow. In addition, Sca-1
+
bone marrow cells also contain the mesenchymal stem cell fraction. Sca-1
+
cells have been shown to give rise to hepatocytes
in vivo
and neural cells
in vitro
. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate.
Additional information: Clone REA422 displays negligible binding to Fc receptors.

Alternative Names

LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Technical specifications

  • Antigen: Sca-1
  • Clone: REA422
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
  • Entrez Gene ID: 110454
  • Molecular mass of antigen [kDa]: 9
  • Distribution of antigen: hematopoietic stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, APC, PE-Vio770, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA422
D7++
E13-161.7++
  • Selected References

    1. Bradfute, S. B. et al. (2005) Roles of Sca-1 in hematopoietic stem/progenitor cell function. Exp. Hematol. 33(7): 836-843
    2. Khan, K. D. et al. (1990) Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells. Mol. Cell. Biol. 10(10): 5150-5159
    3. Houlihan, D. D. et al. (2012) Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nat. Protoc. 7(12): 2103-2111
Product options: 7

Product information

Size
Order no.
Price

Anti-Sca-1-VioBright FITC, mouse

  • REA422
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-259
EUR 43,00 

Anti-Sca-1-APC, mouse

  • REA422
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-428
EUR 43,00 

Anti-Sca-1-APC, mouse

  • REA422
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-425
EUR 80,00 

Anti-Sca-1-PE-Vio770, mouse

  • REA422
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-258
EUR 52,00 

Anti-Sca-1-PE-Vio770, mouse

  • REA422
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-220
EUR 100,00 

Anti-Sca-1-Biotin, mouse

  • REA422
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-106-426
EUR 15,00 

Anti-Sca-1-Biotin, mouse

  • REA422
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-106-423
EUR 40,00