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Human peripheral blood mononuclear cells (PBMCs) from a healthy donor (first column), lysed CLL whole blood sample (second column), or a mixture of human PBMCs and mantle cell lymphoma (MCL) cell line, Jeko-1, (third column) were stained with Anti-ROR1 antibodies as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. PBMCs from a healthy donor are negative for ROR1 expression. Data were gated on CD45+
cells. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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