Anti-PLK1 pT210 antibodies, human

Anti-PLK1 pT210 antibodies, human

Clone: REA530 | Dilution: 1:11
Clone REA530 recognizes the human polo-like kinase (PLK1) antigen phosphorylated at threonine 210 (pT210). PLK1 is a key regulator of several important cell-cycle–associated processes, such as centrosome maturation, spindle assembly, sister chromatid cohesion, cytokinesis, and recovery from a DNA-damage–induced arrest. Expression of PLK1 is highly cell cycle regulated; its expression is first induced in G2 and peaks during the early stages of mitosis. PLK1 has been described to be recruited to the centrosomes in G2, as well as to spindle poles and kinetochores during mitosis. Activation of PLK1 is dependent on phosphorylation of a conserved threonine residue (T210) in the T-loop of its kinase domain. Phosphorylation of this residue starts in G2, when PLK1 activity gradually increases until it reaches its full activity when cells enter mitosis. The initial phosphorylation of T210 in G2 is mediated by the kinase Aurora-A, in concert with its cofactor Bora. Since PLK1 is highly expressed in several carcinomas, and expression is inversely correlated with the survival rate of patients in non-small cell lung, head and neck, and esophageal cancer, PLK1 is recognized as a valid prognostic marker.
Additional information: Clone REA530 displays negligible binding to Fc receptors.

Alternative Names

PLK-1, STPK13

Technical specifications

  • Antigen: PLK1 pT210
  • Clone: REA530
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: PLK-1, STPK13
  • Entrez Gene ID: 5347
  • Molecular mass of antigen [kDa]: 68
  • Distribution of antigen: ubiquitous
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected References

    1. Hamanaka, R. et al. (1994) Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase. Cell Growth Differ. 5(3): 249-257
    2. Bruinsma, W. et al. (2014) Bora and Aurora-A continue to activate Plk1 in mitosis. J. Cell. Sci. 127(4): 801-811
    3. Kakeno, M. et al. (2014) Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment. Cell Struct. Funct. 39(1): 45-59
Product options: 4

Product information

Size
Order no.
Price

Anti-PLK1 pT210-PE, human

  • REA530
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-197
EUR 112,00 

Anti-PLK1 pT210-PE, human

  • REA530
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-171
EUR 296,00 

Anti-PLK1 pT210-APC, human

  • REA530
  • 1:11
  • For research use only
for 30
tests
(1)
130-108-198
EUR 146,00 

Anti-PLK1 pT210-APC, human

  • REA530
  • 1:11
  • For research use only
for 100
tests
(1)
130-108-172
EUR 322,00