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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left peak) or stimulated with 10 µM PMA at 37 °C for 24 hours. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A and stained with Anti-PKCθ antibodies. CD3+
cells were pregated for the analysis. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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