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Human peripheral blood mononuclear cells (PBMCs) were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were stained with Anti-IRF-8 antibodies or with the corresponding isotype control antibodies (left image) as well as with CD123 antibodies. CD14-
cells were pregated for the analysis. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals.
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