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Thioglycolate-elicited peritoneal macrophages from BALB/c mice were stimulated for 5 hours with 100 ng/mL LPS and 1 µg/mL brefeldin A at 37 °C. Cells were then fixed, permeabilized, and stained with Anti-IL-1β Pro-form antibodies or with the corresponding REA Control antibodies (left image) as well as with CD11b antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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