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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with LPS/R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were then stained with CD14 antibodies and fixed, permeabilized, and intracellularly stained with Anti-IL-12 (p35/p70) antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.