Anti-F4/80 antibodies, mouse

Anti-F4/80 antibodies, mouse

Clone: REA126 | Dilution: 1:10
Clone REA126 recognizes F4/80, a member of epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 is considered as one of the most specific cell-surface markers for murine macrophages. F4/80 is a seven-span transmembrane molecule with a large extracellular, multiple EGF module–containing domain. Constitutive and high expression of F4/80 is found on most resident tissue macrophages, including the spleen, microglia in the brain, Kupffer's cells in the liver, and Langerhans cells in the skin. In addition, the expression of F4/80 can also be regulated depending on the physiological status of the cell. F4/80 molecule is required for the differentiation of antigen-specific CD8
+
Tregs and is involved in inducing peripheral immune tolerance. No specific ligand for F4/80 has been reported so far.
Additional information: Clone REA126 displays negligible binding to Fc receptors.

Alternative Names

ADGRE1, DD7A5-7, EGF-TM7, EMR1, Gpf480, Ly71, TM7LN3

Technical specifications

  • Antigen: F4/80
  • Clone: REA126
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: ADGRE1, DD7A5-7, EGF-TM7, EMR1, Gpf480, Ly71, TM7LN3
  • Entrez Gene ID: 13733
  • Molecular mass of antigen [kDa]: 99
  • Distribution of antigen: macrophages
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE-Vio615, Biotin
  • Selected References

    1. Lin, H-H. et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. J. Exp. Med. 201: 1615-1625
    2. Gordon, S. et al. (2011) F4/80 and the related adhesion-GPCRs. Eur. J. Immunol. 41(9): 2472-2476
    3. Kortlever, R. et al. (2017) Myc Cooperates with Ras by Programming Inflammation and Immune Suppression. Cell 171(6)
    4. Hume, D. A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80: macrophages of bone and associated connective tissue. J. Cell. Sci. 66: 189-194
    5. Gu, C. et al. (2018) EcoHIV infection of mice establishes latent viral reservoirs in T cells and active viral reservoirs in macrophages that are sufficient for induction of neurocognitive impairment. PLoS Pathog. 14(6)
    6. Siegmund, D. et al. (2016) Activation of TNFR2 sensitizes macrophages for TNFR1-mediated necroptosis. Cell Death Dis 7(9): e2375
    7. Siegmund, D. et al. (2018) TNFR2 unlocks a RIPK1 kinase activity-dependent mode of proinflammatory TNFR1 signaling. Cell Death Dis 9(9)
    8. Lee, H. et al. (2017) Kinetics of Corneal Antigen Presenting Cells in Experimental Dry Eye Disease. BMJ Open Ophthalmol 1(1)
    9. Jia, J. et al. (2018) Cholesterol metabolism promotes B-cell positioning during immune pathogenesis of chronic obstructive pulmonary disease. EMBO Mol. Med. 10(5)
Product options: 4

Product information

Size
Order no.
Price

Anti-F4/80-PE-Vio615, mouse

  • REA126
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-744
EUR 67,00 

Anti-F4/80-PE-Vio615, mouse

  • REA126
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-709
EUR 101,00 

Anti-F4/80-Biotin, mouse

  • REA126
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-102-029
EUR 15,00 

Anti-F4/80-Biotin, mouse

  • REA126
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-101-893
EUR 32,00 

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