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Human peripheral blood mononuclear cells (PBMCs) were stimulated with CD3 and CD28 antibodies for 3 days. Cells were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with Anti-BATF antibodies or with the corresponding REA Control (I) antibodies (left peak). Flow cytometry was performed using the MACSQuant®
Analyzer. Lymphocytes were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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