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Human Jurkat cells were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-AKT pS473 antibodies or with the corresponding REA Control (I) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris was excluded from the analysis based on scatter signals.
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