Anti-A2B5 antibodies, human, mouse, rat

Anti-A2B5 antibodies, human, mouse, rat

Clone: 105HB29 | Dilution: 1:11
Clone 105HB29 recognizes the c-series ganglioside–specific antigen A2B5. The Anti-A2B5 antibody binds to glial progenitors from embryonic to adult human, murine, and rat tissue.
A2B5 is predominantly expressed in embryonic and neonatal neural tissue. In the adult mammalian brain, A2B5 expression is restricted mainly to areas which retain neurogenic potential such as the subventricular zone (SVZ). Thus, A2B5 is considered as a marker for immature glial-committed precursors which are permanently generated in the SVZ. Glial precursor cells are defined as cells that give rise to glial cell types, such as astrocytes and oligodendrocytes.
Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides, and both antigens are down-regulated as the cells differentiate into mature oligodendrocytes. Therefore, A2B5 serves as a marker to monitor the maturation of oligodendrocyte progenitors in oligodendrocyte cultures.

Technical specifications

  • Antigen: A2B5
  • Clone: 105HB29
  • Isotype: mouse IgMκ
  • Isotype control: Mouse IgM – isotype control antibodies
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, Biotin, pure, PE
  • Selected references

    1. Dietrich, J. et al. (2002)
      Characterization of A2B5
      glial precursor cells from cryopreserved human fetal brain progenitor cells.
      Glia 40: 65-77
    2. Dubois, C. et al. (1990) Monoclonal antibody A2B5, which detects cell surface antigens, binds to ganglioside GT3 (II3 (NeuAc)3LacCer) and to its 9-O-acetylated derivative. J. Biol. Chem. 265: 2797-2803
    3. Larsen, P. H. et al. (2003) Matrix metalloproteinase-9 facilitates remyelination in part by processing the inhibitory NG2 proteoglycan. J. Neurosci. 23: 11127-11135
    4. Ruffini, F. et al. (2004) Distinctive properties of human adult brain-derived myelin progenitor cells. Am. J. Pathol. 165: 2167-2175
    5. Windrem, M. (2004) Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat. Med. 10: 93-97
    6. Larsen, P. H. and Yong, V. W. (2004) The expression of matrix metalloproteinase-12 by oligodendrocytes regulates their maturation and morphological differentiation. J. Neurosci. 24: 7597-7603
    7. Larsen, P. H. et al. (2006) Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J. Neurosci. 26: 2207-2214
    8. Windrem, M. et al. (2004) Corrigendum: Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat. Med. 10: 209
    9. Najm, F. J. et al. (2013) Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat. Biotechnol. 31(5): 426-433
    10. Albert, M. et al. (2013) The histone demethylase Jarid1b ensures faithful mouse development by protecting developmental genes from aberrant H3K4me3. PLoS Genet. 9(4): e1003461
  • Brochures and posters

Product options: 5
for 30
EUR 113,00 
for 100
EUR 273,00 
for 30
EUR 93,00 
for 100
EUR 227,00 
100 µg in 1 mL
EUR 178,00 

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