StraightFrom™ Whole Blood CD3 MicroBeads, human

StraightFrom™ Whole Blood CD3 MicroBeads, human

StraightFrom™ Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom™ Whole Blood CD3 MicroBeads recognize the CD3 antigen which is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes. StraightFrom Whole Blood MicroBeads allow the fast isolation of CD3
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom™ Whole Blood CD3 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

The purified CD3
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1

Product information

Size
Order no.
Price

StraightFrom Whole Blood CD3 MicroBeads, human

  • For research use only
for 40
mL whole blood
130-090-874
EUR 653,00 

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