StraightFrom™ Whole Blood CD138 MicroBeads, human

StraightFrom™ Whole Blood CD138 MicroBeads, human

StraightFrom™ Whole Blood CD138 MicroBeads were developed for rapid positive selection of CD138
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis.

Background information

The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. In peripheral blood of healthy donors approximately 50% of plasma cells express this antigen. In contrast, CD138 is not expressed by naive B cells, germinal center B cells, memory B cells, T cells, and monocytes.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom™ Whole Blood CD138 MicroBeads and subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit. StraightFrom Whole Blood MicroBeads in combination with the autoMACS Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.

Downstream applications

The purified CD138
+
cells are well suited for flow cytometric, functional, or molecular analysis.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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Product information

Size
Order no.
Price

StraightFrom Whole Blood CD138 MicroBeads, human

  • For research use only
for 40
mL whole blood
130-093-062
EUR 653,00 

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