CD8a+ T Cell Isolation Kit, mouse

CD8a
+
T Cell Isolation Kit
, mouse

The CD8a
+
T Cell Isolation Kit, mouse was developed for the untouched isolation of CD8a
+
T cells from single-cell suspensions from mouse spleen or lymph node. Purity has been improved with the release of this kit due to the addition of TCRγ/δ antibody to the depletion cocktail.
This kit uses the new fast mouse protocol and replaces the CD8a
+
T Cell Isolation Kit II (# 130-095-236).

Background information

The kit is based on a cocktail of biotin-conjugated antibodies against CD4, CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, Ter-119 and TCRγ/δ. The isolation of T cells is achieved by depletion of magnetically labeled cells.

Downstream applications

Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The CD8a
+
T Cell Isolation Kit has been used for the following applications: For adoptive transfer experiments
1,2
, T cell proliferation assays
2,3
, Coculture with dendritic cells
2
and Cytokine analysis experiments.

Columns

LS or autoMACS
®
Columns.
  • Selected references

    1. Hamilton-Williams, E. E. et al. (2009) Expression of diabetes-associated genes by dendritic cells and CD4 T cells drives the loss of tolerance in nonobese diabetic mice. J. Immunol. 183: 1533-1541
    2. Grebe, K. M. et al. (2009)
      Sympathetic nervous system control of anti-influenza CD8
      +
      T cell responses.
      Proc. Natl. Acad. Sci. U.S.A. 106: 5300-5305
    3. Simma et al. (2009) Identification of an indispensable role for tyrosine kinase 2 in CTL-mediated tumor surveillance. Cancer Res. 69: 203-211
Product options: 1
for 1×
10
9
total cells
EUR 609,00 

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