CD8a MicroBeads, rat

CD8a MicroBeads, rat

Rat CD8a MicroBeads have been developed for positive selection or depletion of cytotoxic T cells from lymphoid organs, blood, or other tissues.

Background information

CD8a MicroBeads recognize the immunoglobulin-like domain of the CD8α chain, which is expressed as a heterodimer with CD8β on TCRα/β
+
and TCRγ/δ
+
cytotoxic T cells and on most thymocytes.
As a homodimer of two CD8α chains, the CD8 antigen is expressed on most NK cells,
a major fraction of intestinal intraepithelial lymphocytes, on some activated CD4
+
CD8
+
double-positive T cells, and on CD8
+
T cells from athymic rats. CD8a is also expressed by a T cell subset with regulatory functions.

Downstream applications

Thymocytes or mature T cells isolated with CD8a MicroBeads can be used for
in vitro
analysis
1
or
in vivo
reconstitution
2–4
of T cells, MHC restriction, tolerance induction, and cytokine expression experiments. Functionality of the isolated cells has been shown by several adoptive transfer experiments. Transfer of isolated CD4
+
and CD8a
+
T cells into irradiated recipients results in donor-specific acceptance to heart transplants
2
or type V collagen-induced tolerance to lung allografts
3
. Furthermore, autoimmunity and induction of tolerance after xenogeneic thymus transplantation was studied by adoptive transfer of T cells separated by MACS
®
Technology.
4

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Lombardi, M. S. et al. (2001) Adjuvant arthritis induces down-regulation of G protein-coupled receptor kinases in the immune system. J. Immunol. 166: 1635-1640
    2. Kataoka, M. et al. (2003) Development of infectious tolerance after donor-specific transfusion and rat heart transplantation. J. Immunol. 171: 204-211
    3. Mizobuchi, T. et al. (2003) Differential expression of Smad7 transcripts identifies the CD4+CD45RChigh regulatory T cells that mediate type V collagen-induced tolerance to lung allografts. J. Immunol. 171: 1140-1147
    4. Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J. Immunol. 170: 5936-5946
Product options: 1
for 1×
10
9
total cells
EUR 697,00