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Both the CD56+
NK cell subsets were isolated from human PBMCs using the CD56+
NK Cell Isolation Kit. Non-NK cells were depleted using an LS Column. The enriched NK cells were magnetically labeled with CD8 MicroBeads, and CD56+
NK cells were isolated by positive selection using an MS Column. For isolation of the CD56+
NK cells, the wash and the flow-through fraction of the MS Column were further separated using an LD Column and collected in the flow-through. Cells were fluorescently stained with CD8-PE (clone 3G8) and CD56-APC.
PBMCs before separation
Pre-enriched NK cells after depletion of non-NK cells
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