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monocytes were isolated from PBMCs using the CD16+
Monocyte Isolation Kit. In a first step, granulocytes and NK cells were depleted. In a second step, CD16+
cells were enriched. An LD Column and a MidiMACS™ Separator were used for the depletion step, and an MS Column and a MiniMACS™ Separator for the positive selection step.
PBMCs before separation
T cell–depleted flow through
Isolated Slan (M-DC8)+
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