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Isolation of lineage marker-negative cells from mouse bone marrow using the Direct Lineage Cell Depletion Kit, a MidiMACS™ Separator and an LS Column. Cells were fluorescently labeled with the Lineage Cell Detection Cocktail-Biotin and Anti-Biotin-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. In order to evaluate the LSK fraction, cells were additionally labeled with CD117-PE and Anti-SCA-1-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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