Diamond CD133 Isolation Kit, human

Diamond CD133 Isolation Kit, human

The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin
CD133
+
stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.

Detailed separation procedure

The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133
+
cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin
-
CD133
+
stem cells.
Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.

Downstream applications

Lin
-
CD133
+
stem cells can be used for many applications, including:
  • gene or miRNA expression profiling
  • characterization of undifferentiated stem cells and their developmental potential
  • stimulation of proliferation and maturation by cytokines
  • HSC-CFU assays
  • adoptive transfer and hematopoietic reconstitution studies

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
Product options: 1

Product information

Size
Order no.
Price

Diamond CD133 Isolation Kit, human

  • For research use only
for 2×
10
9
total cells
130-094-913
EUR 815,00 

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