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Miltenyi Biotec Inc.
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Phone: +1 800 FOR MACS
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Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence. |
A: | B: |
Unseparated fraction | After separation |
 |  |
Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells. |
A: | B: |
Without separation | With separation |
 |  |
