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Technical support - Clinical products
SSEA-4 expressing cells were isolated from a heterogeneous suspension of U937 cells (SSEA-4–
) and NTERA cells (SSEA-4+
) using Anti-SSEA-4 MicroBeads, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Labeling Check Reagent-FITC and CD326 (EpCAM)-APC. Cell debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.