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DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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