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Miltenyi Biotec B.V.
Schipholweg 68 H
2316 XE Leiden
The Netherlands
Phone: 800 24971
Fax: 800 24984
E-Mail: macs@miltenyibiotec.nl
Web: www.miltenyibiotec.com
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Technical support - Clinical products
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E-Mail: macs@miltenyibiotec.nl
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
PBMCs from a CMV + donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4 + T cells were detected per 10 6 CD4 + T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment. |
Stimulated sample | |
Before enrichment | After enrichment |
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Unstimulated control | |
Before enrichment | After enrichment |
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