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Cardiac fibroblasts were isolated from P2 CD-1R mouse hearts by using the Neonatal Cardiac Fibroblast Isolation Kit, two MS Columns, and a MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuantR Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.