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T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress®
Whole Blood CD8 T Cell Isolation Kit, a MACSmix Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45- VioBlue®
, CD3-APC, CD8-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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