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Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602
USA
Phone: +1 800 FOR MACS
+1 530 888 8871
Fax: +1 530 888 8925
E-Mail: macs@miltenyibiotec.com
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Phone: +1 866 811 4466
Fax: +1 877 591 1060
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Technical support - Research products
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
Technical support - Clinical products
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
With MACSxpress® Technology, direct isolation of immune cells from human blood has never been easier. It allows processing of large blood volumes of up to 30 mL in one single tube. Without the need for density gradient centrifugation, this technology offers a fast and easy way to isolate viable and functional immune cells from various blood products.
Using one of our MACSxpress Isolation Kits, you get highly pure target cells within 25 minutes. In this example, neutrophils were isolated from whole blood using the MACSxpress Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator.
Untouched neutrophils were isolated from 8 mL of human EDTA anticoagulated whole blood using the MACSxpress® Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD14-PerCP, CD15-PE, CD16-APC, CD45-VioBlue®, and CD193-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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