ODN 2216

ODN 2216

Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 2216 is an A-class ODN for activation of pDCs, induction of IFN-α secretion from PBMCs, and activation of IFN signalling pathways.

Background information

TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells
1
,
2
. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs
3
.
Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.

Applications

A-class ODN for activation of pDCs, induction of IFN-α secretion from human PBMCs, and activation of IFN signalling pathways.
A-class ODN form tertiary structure (G tetrads).
  • Selected References

    1. Hemmi, H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
    2. Krieg, A. M. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
    3. Bauer, S. et al. (2001) Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc. Natl. Acad. Sci. U.S.A. 98: 9237-9242
Product options: 2
130-100-243

ODN 2216

200 µg
EUR 177,00 
130-100-244

ODN 2216

1 mg
EUR 615,00