MSCs were isolated from human bone marrow and expanded with MACS NH Expansion Medium. After two passages MSCs were co-cultured with CD4+
responder T cells (Tresp) at different ratios. For T cell stimulation, the MSC Suppression Inspector was added to the culture. Proliferation of T cells was determined by3
Tresp show high proliferation after stimulation with the MSC Suppression Inspector (A). When adding MSCs, Tresp proliferation is suppressed dramatically (B). Unstimulated Tresp show no proliferation (D). MSCs alone show little proliferation with or without stimulation (C and E).