PepTivator gp100/Pmel 17, human

PepTivator gp100/Pmel 17, human

The PepTivator
®
gp100/Pmel 17 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acids (aa) overlap, covering the complete sequence of this antigen (UniProt ID: P40967).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells, as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of gp100/Pmel 17–specific T cell immunity can provide important information on the natural course of immune responses.

Background information

Gp100/Pmel 17 belongs to the melanoma-associated antigens of the group of differentiation antigens. Other members of this group are, for example, tyrosinase and Melan-A/MART-1. Gp100/Pmel 17 is expressed on normal melanocytes, but is also present in melanoma. CD4
+
and CD8
+
T lymphocytes recognizing gp100/Pmel 17 have been identified in melanoma patients.
2

Downstream applications

The
in vitro
stimulation of gp100/Pmel 17 CD4
+
and CD8
+
T cells with PepTivator gp100/Pmel 17 causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of gp100/Pmel 17–specific T cells.
  • Detection and analysis of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable gp100/Pmel 17–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells for research on immunotherapy and vaccination
  • Pulsing of antigen-presenting cells, for example, for research in dendritic cell vacchination..
Product options: 2

Product information

Size
Order no.
Price

PepTivator gp100/Pmel 17 - premium grade, human

  • For research use only
for
stimulation of
10
8
cells
130-094-449
EUR 213,00 

PepTivator gp100/Pmel 17 - premium grade, human

  • For research use only
for
stimulation of
10
9
cells
130-094-450
EUR 1.246,00 

Related products

2 products available