PepTivator Dengue Virus Type 2 Envelope Protein E1, human

PepTivator Dengue Virus Type 2 Envelope Protein E1, human

The PepTivator
Dengue Virus Type 2 Envelope Protein E1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Dengue Virus Type 2 Envelope Protein E1. The sequence is derived from UniProt Acc. no. P14340 and covers aa 281–535 of the genome polyprotein.
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

The Dengue virus can be found in tropical and subtropical areas worldwide and belongs to the family of
. Dengue fever is a mosquito transmitted viral infection and can be caused by four distinct serotypes (DEN-1, DEN-2, DEN-3, and DEN-4), inducing high fever, severe muscle pain, and nausea. Dengue virus type 2 (DENV-2) is the most common infecting serotype. Recovery from infection by one Dengue virus induces immunity against the homologous serotype but not against the other three serotypes. Secondary infection with a different DENV serotype can cause the potentially life-threatening dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS).

Downstream applications

Dengue Virus Type 2 Envelope Protein E1 - research grade has been specifically developed for efficient
in vitro
stimulation of PepTivator Dengue–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
and CD8
T cells in various applications, including:
  • Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable antigen-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of antigen-specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
Product options: 1
stimulation of
EUR 141,00